酯香香

您现在所在的位置 : 资讯中心 > 技术解答 > 上海药物所徐华强课题组利用冷冻电镜技术揭示抗偏头痛药物选择性作用机制

上海药物所徐华强课题组利用冷冻电镜技术揭示抗偏头痛药物选择性作用机制

阅读量：240

发布时间：2021-10-14

来源：酯香香化学采购网

　　【化工仪器网项目成果】偏头痛是一种影响极为广泛的神经系统疾病，在全球范围内波及超过10亿人口，造成了巨大的社会经济负担。据统计，欧洲每年因偏头痛造成超过270亿欧元的经济损失，在中国约每11个成人中就有1人遭受偏头痛的困扰。此外，偏头痛还会伴随包括抑郁症、焦虑症、癫痫、肥胖和其它慢性疼痛等一系列病症，给患者及其家庭带来沉重负担。

　　5-羟色胺(5-HT)家族受体是偏头痛、抑郁症、精神分裂症等中枢神经疾病的重要靶点。其中，5-HT1B、5-HT1D和5-HT1F三种亚型与偏头痛的治疗密切相关。多年以来，靶向5-HT1B/1D的激动剂曲普坦类药物被广泛用于偏头痛的治疗。然而，该类药物的血管收缩特性给患有冠心病、脑血管疾病或高血压病史的患者带来了一定的治疗风险。2019年，美国FDA批准了一种高选择性靶向5-HT1F的新型急性偏头痛治疗药物——拉米替坦(Lasmiditan)。拉米替坦能有效地避免曲普坦类药物在心血管方面的副作用，然而其选择性靶向5-HT1F受体的机理尚不明确。5-HT1F作为极具前景的抗偏头痛靶点，对其结构、功能以及选择性药物的作用机制的研究具有重要意义。

　　近日，中国科学院上海药物研究所徐华强课题组利用冷冻电镜技术，首次解析了5-HT1F受体结合G蛋白以及抗偏头痛药物拉米替坦的复合物结构，揭示了拉米替坦选择性结合5-HT1F受体的结构基础。

　　冷冻电镜技术，也叫冷冻电子显微镜技术，是在低温下使用透射电子显微镜观察样品的显微技术，即把样品冻起来并保持低温放进显微镜里面，用高度相干的电子作为光源从上面照下来，透过样品和附近的冰层，受到散射。研究人员再利用探测器和透镜系统把散射信号成像记录下来，最后进行信号处理，得到样品的结构。

　　冷冻电镜技术作为一种重要的结构生物学研究方法，它与X射线晶体学、核磁共振一起构成了高分辨率结构生物学研究的基础。这项技术获得了2017年的诺贝尔化学奖。获奖理由是“开发出冷冻电子显微镜技术(也称为低温电子显微镜技术)用于确定溶液中的生物分子的高分辨率结构”，简化了生物细胞的成像过程，提高了成像质量。

　　徐华强课题组的成果以“Structural basis for recognition of anti-migraine drug lasmiditan by the serotonin receptor 5-HT1F–G protein complex”为题，于2021年7月8日在《细胞研究》(Cell Research)上在线发表。

　　5-HT1F属于5-HT1亚家族成员，但在同源性和配体激活效应上与该亚家族的其它亚型差别相对较大，这也使得5-HT1F成为具有潜力的选择性抗偏头痛靶点。研究团队经过纯化、冷冻制样和数据处理等条件摸索，突破了5-HT1F受体-G蛋白复合物表达量低、复合物组装不稳定的技术瓶颈，最终获得高质量的复合物结构。5-HT1F受体的胞外区附近结构相对其他5-HT亚型受体具有显著的构象变化，这也是药物拉米替坦能够高选择性结合5-HT1F受体的结构基础。

　　a-b. 5-HT1F-Gi-拉米替坦复合物的电镜密度图(a)和原子模型(b)；
c. 拉米替坦的结合口袋示意图；
d. 拉米替坦与5-HT1F受体的相互作用模式图；
e. G蛋白招募实验显示拉米替坦对5-HT1F受体具有高度选择性。

　　徐华强课题组长期致力于在5-羟色胺家族受体的结构与功能研究，并取得了一系列系统性的重要成果。该研究团队于2013年在Science上发表首个5-HT1B受体的晶体结构1；于2018年在Cell Discovery上发表了首个拮抗状态的5-HT1B受体结构2；于2021年3月在Nature上发表3个不同亚型的5-HT受体与G蛋白复合物的冷冻电镜结构，并首次揭示了5-HT受体的脂质调控、组成型激活以及与抗精神分裂症、抗抑郁药物阿立哌唑的作用机制3。该团队在5-HT1F受体和抗偏头痛药物的作用机制上取得的成果，进一步实现了5-HT受体系统研究领域的重要突破。

　　上海药物所和上海科技大学联合培养博士生黄思婕、上海药物所博士生徐沛雨和研究助理谭阳霞为文章的共同第一作者；上海药物所徐华强研究员和蒋轶研究员为文章的共同通讯作者。该研究获得了国家重点研发计划、中国科学院战略性先导科技专项、上海市市级科技重大专项、国家自然科学基金和国家科技重大专项的资助。

内容来源于酯香香化学采购网

下一篇：多参数测量水下环视摄像海洋观测又有新设备

上一篇：加拿大油菜籽压榨步伐强劲同比增长近5个百分点

版权申明：

1.凡本网注明“来源：酯香香化学采购网”的所有文字、图片、音频和视频文件，版权均为有料网所有。如需转载请与客服电话：4008-938-935联系。任何媒体、网站或个人转载使用时须注明来源“有料网”，违反者本网将追究法律责任。
2.本网转载并注明其他来源的稿件，均来自互联网或业内投稿人士，版权属于原版权人。转载请保留稿件来源及作者，禁止擅自篡改违者自负版权法律责任。
3.本网评论版权中各网友的评论只代表网友个人观点，不代表本站的观点或立场。

产品品鉴

生物医用酯

酯类增塑剂

基础油脂

化工资讯

我国天然气产量连续3年增产超100亿立方米 2020中国（河北）国际橡胶塑料产业博览会火热启动 2020上海国际金属加工液、润滑油展览会火热启动 OPEC迎十年最大减产明年油价或继续承压 “智能+绿色”成化工教育重要方向污水处理迎来快速增长期化工人才需求上涨11.5%

行业新闻

加拿大油菜籽压榨步伐强劲同比增长近5个百分点 Nornickel将污染严重的铜冶炼厂关闭以推动环保财务省：日本11月精炼铜量同比增长37.4 中国主要铜冶炼厂下调2021年一季度的TC/RC 托管太钢集团，宝武系A股方阵扩容至10家中国进口澳大利亚铜精矿数量降至2016年以来最低水平

基本文件流程错误 SQL 调试

/www/dev/zhihuagong/public/index.php ( 1.51 KB )
/www/dev/zhihuagong/thinkphp/base.php ( 1.71 KB )
/www/dev/zhihuagong/thinkphp/library/think/Loader.php ( 12.70 KB )
/www/dev/zhihuagong/vendor/composer/autoload_static.php ( 21.91 KB )
/www/dev/zhihuagong/thinkphp/library/think/Error.php ( 4.00 KB )
/www/dev/zhihuagong/vendor/psr/log/Psr/Log/LoggerInterface.php ( 3.04 KB )
/www/dev/zhihuagong/thinkphp/library/think/Container.php ( 17.20 KB )
/www/dev/zhihuagong/thinkphp/library/think/App.php ( 27.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/Env.php ( 2.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/Config.php ( 9.71 KB )
/www/dev/zhihuagong/thinkphp/convention.php ( 11.74 KB )
/www/dev/zhihuagong/application/tags.php ( 1.08 KB )
/www/dev/zhihuagong/thinkphp/library/think/Hook.php ( 5.58 KB )
/www/dev/zhihuagong/application/common/behavior/Config.php ( 1.68 KB )
/www/dev/zhihuagong/application/common.php ( 32.83 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Request.php ( 6.32 KB )
/www/dev/zhihuagong/thinkphp/library/think/Facade.php ( 3.44 KB )
/www/dev/zhihuagong/thinkphp/library/think/Request.php ( 57.54 KB )
/www/dev/zhihuagong/application/function.php ( 0.68 KB )
/www/dev/zhihuagong/thinkphp/helper.php ( 20.40 KB )
/www/dev/zhihuagong/config/addons.php ( 1.33 KB )
/www/dev/zhihuagong/config/app.php ( 6.17 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Env.php ( 1.17 KB )
/www/dev/zhihuagong/config/cache.php ( 1.59 KB )
/www/dev/zhihuagong/config/captcha.php ( 0.96 KB )
/www/dev/zhihuagong/config/constant.php ( 1.33 KB )
/www/dev/zhihuagong/config/cookie.php ( 1.12 KB )
/www/dev/zhihuagong/config/database.php ( 2.36 KB )
/www/dev/zhihuagong/config/info.php ( 0.82 KB )
/www/dev/zhihuagong/config/log.php ( 1.01 KB )
/www/dev/zhihuagong/config/session.php ( 1.06 KB )
/www/dev/zhihuagong/config/template.php ( 2.24 KB )
/www/dev/zhihuagong/config/token.php ( 1.43 KB )
/www/dev/zhihuagong/config/trace.php ( 0.84 KB )
/www/dev/zhihuagong/config/version.php ( 0.92 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-php80/bootstrap.php ( 1.46 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-mbstring/bootstrap.php ( 7.07 KB )
/www/dev/zhihuagong/vendor/symfony/var-dumper/Resources/functions/dump.php ( 0.79 KB )
/www/dev/zhihuagong/vendor/symfony/deprecation-contracts/function.php ( 0.98 KB )
/www/dev/zhihuagong/vendor/ralouphie/getallheaders/src/getallheaders.php ( 1.60 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/psr7/src/functions_include.php ( 0.15 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/psr7/src/functions.php ( 13.09 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-intl-normalizer/bootstrap.php ( 0.71 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-php72/bootstrap.php ( 1.89 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/promises/src/functions_include.php ( 0.16 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/promises/src/functions.php ( 9.89 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-intl-idn/bootstrap.php ( 4.52 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/guzzle/src/functions_include.php ( 0.16 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/guzzle/src/functions.php ( 9.70 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-ctype/bootstrap.php ( 1.56 KB )
/www/dev/zhihuagong/vendor/khanamiryan/qrcode-detector-decoder/lib/common/customFunctions.php ( 1.75 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/helpers.php ( 3.61 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/alias.php ( 1.21 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Arrayable.php ( 0.19 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Jsonable.php ( 0.24 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Htmlable.php ( 0.19 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Arr.php ( 16.36 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Traits/Macroable.php ( 2.63 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Collection.php ( 33.51 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Traits/EnumeratesValues.php ( 26.07 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Enumerable.php ( 23.81 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/HigherOrderCollectionProxy.php ( 1.40 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/HigherOrderWhenProxy.php ( 1.30 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/LazyCollection.php ( 33.64 KB )
/www/dev/zhihuagong/vendor/overtrue/pinyin/src/const.php ( 0.49 KB )
/www/dev/zhihuagong/vendor/topthink/think-captcha/src/helper.php ( 1.54 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Route.php ( 3.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/Route.php ( 25.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Domain.php ( 7.08 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleGroup.php ( 16.45 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Rule.php ( 28.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleItem.php ( 8.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleName.php ( 3.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Validate.php ( 4.83 KB )
/www/dev/zhihuagong/thinkphp/library/think/Validate.php ( 43.93 KB )
/www/dev/zhihuagong/vendor/topthink/think-helper/src/helper.php ( 7.35 KB )
/www/dev/zhihuagong/vendor/overtrue/wechat/src/Kernel/Support/Helpers.php ( 2.54 KB )
/www/dev/zhihuagong/vendor/overtrue/wechat/src/Kernel/Helpers.php ( 1.89 KB )
/www/dev/zhihuagong/thinkphp/library/think/Db.php ( 7.67 KB )
/www/dev/zhihuagong/thinkphp/library/think/Lang.php ( 7.38 KB )
/www/dev/zhihuagong/thinkphp/library/think/Log.php ( 8.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/log/driver/File.php ( 8.97 KB )
/www/dev/zhihuagong/thinkphp/lang/zh-cn.php ( 12.58 KB )
/www/dev/zhihuagong/route/route.php ( 0.67 KB )
/www/dev/zhihuagong/route/route_cms.php ( 2.89 KB )
/www/dev/zhihuagong/route/router_api.php ( 0.84 KB )
/www/dev/zhihuagong/route/router_h5.php ( 0.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Config.php ( 1.52 KB )
/www/dev/zhihuagong/extend/libs/Cache_factory.php ( 2.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Cache.php ( 2.06 KB )
/www/dev/zhihuagong/thinkphp/library/think/Cache.php ( 3.27 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/driver/File.php ( 7.99 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/Driver.php ( 8.64 KB )
/www/dev/zhihuagong/application/common/behavior/InitHook.php ( 1.47 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Hook.php ( 1.46 KB )
/www/dev/zhihuagong/addons/phpmailer/Phpmailer.php ( 2.00 KB )
/www/dev/zhihuagong/extend/sys/Addons.php ( 6.49 KB )
/www/dev/zhihuagong/thinkphp/library/think/View.php ( 5.74 KB )
/www/dev/zhihuagong/thinkphp/library/think/view/driver/Think.php ( 6.12 KB )
/www/dev/zhihuagong/thinkphp/library/think/Template.php ( 47.31 KB )
/www/dev/zhihuagong/thinkphp/library/think/template/driver/File.php ( 2.29 KB )
/www/dev/zhihuagong/application/cms/behavior/Hooks.php ( 1.10 KB )
/www/dev/zhihuagong/extend/sys/Hooks.php ( 2.48 KB )
/www/dev/zhihuagong/application/cms/function.php ( 10.81 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/dispatch/Module.php ( 5.19 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Dispatch.php ( 9.26 KB )
/www/dev/zhihuagong/application/cms/config/paginate.php ( 0.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/Middleware.php ( 5.34 KB )
/www/dev/zhihuagong/thinkphp/library/think/Cookie.php ( 7.35 KB )
/www/dev/zhihuagong/thinkphp/library/think/Session.php ( 14.13 KB )
/www/dev/zhihuagong/thinkphp/library/think/Debug.php ( 7.57 KB )
/www/dev/zhihuagong/application/cms/controller/Article.php ( 3.50 KB )
/www/dev/zhihuagong/application/cms/controller/Cmsbase.php ( 11.29 KB )
/www/dev/zhihuagong/application/member/controller/MemberBase.php ( 2.70 KB )
/www/dev/zhihuagong/application/common/controller/Homebase.php ( 2.38 KB )
/www/dev/zhihuagong/application/common/controller/Base.php ( 0.87 KB )
/www/dev/zhihuagong/thinkphp/library/think/Controller.php ( 7.53 KB )
/www/dev/zhihuagong/thinkphp/library/traits/controller/Jump.php ( 4.76 KB )
/www/dev/zhihuagong/application/member/service/User.php ( 13.62 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Cookie.php ( 1.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Url.php ( 1.13 KB )
/www/dev/zhihuagong/thinkphp/library/think/Url.php ( 13.22 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/driver/Redis.php ( 7.24 KB )
/www/dev/zhihuagong/application/admin/model/NavCategory.php ( 0.09 KB )
/www/dev/zhihuagong/application/admin/model/Base.php ( 4.15 KB )
/www/dev/zhihuagong/thinkphp/library/think/Model.php ( 28.52 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/Attribute.php ( 17.64 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/RelationShip.php ( 20.60 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/ModelEvent.php ( 6.04 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/TimeStamp.php ( 2.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/Conversion.php ( 7.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Session.php ( 2.05 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Connection.php ( 58.93 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/connector/Mysql.php ( 6.22 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/builder/Mysql.php ( 5.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Builder.php ( 36.76 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Query.php ( 105.00 KB )
/www/dev/zhihuagong/extend/util/Tree.php ( 15.34 KB )
/www/dev/zhihuagong/application/cms/service/GoodsService.php ( 14.62 KB )
/www/dev/zhihuagong/application/cms/service/Base.php ( 0.62 KB )
/www/dev/zhihuagong/application/common/model/Goods.php ( 0.08 KB )
/www/dev/zhihuagong/application/cms/service/ComponentsService.php ( 12.91 KB )
/www/dev/zhihuagong/application/admin/model/Config.php ( 3.34 KB )
/www/dev/zhihuagong/application/links/model/Links.php ( 0.84 KB )
/www/dev/zhihuagong/application/admin/model/Poster.php ( 0.07 KB )
/www/dev/zhihuagong/application/admin/model/GoodsCategory.php ( 0.09 KB )
/www/dev/zhihuagong/thinkphp/library/think/exception/ErrorException.php ( 1.68 KB )
/www/dev/zhihuagong/thinkphp/library/think/Exception.php ( 1.61 KB )
/www/dev/zhihuagong/thinkphp/library/think/exception/Handle.php ( 8.96 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Expression.php ( 1.11 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/Collection.php ( 3.23 KB )
/www/dev/zhihuagong/thinkphp/library/think/Collection.php ( 13.60 KB )
/www/dev/zhihuagong/runtime/temp/b275df7f7078649353258fc8c4a5003e.php ( 37.86 KB )
/www/dev/zhihuagong/thinkphp/library/think/Response.php ( 9.68 KB )
/www/dev/zhihuagong/thinkphp/library/think/debug/Html.php ( 4.02 KB )

[ DB ] CONNECT:[ UseTime:0.000505s ] mysql:host=127.0.0.1;port=3306;dbname=yzncms;charset=utf8
[ SQL ] SHOW COLUMNS FROM `yzn_nav_category` [ RunTime:0.002809s ]
[ SQL ] SELECT * FROM `yzn_nav_category` WHERE `status` = 1 ORDER BY `listorder` ASC [ RunTime:0.000629s ]
[ SQL ] SHOW COLUMNS FROM `yzn_poster` [ RunTime:0.001225s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 99 AND `p_c_id` = 52 AND `status` = 1 LIMIT 1 [ RunTime:0.000479s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 99 AND `p_c_id` = 55 AND `status` = 1 LIMIT 1 [ RunTime:0.000394s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods_property` [ RunTime:0.001281s ]
[ SQL ] SELECT `g`.`name`,`p`.`price`,`p`.`unit`,`p`.`type`,`p`.`thumb`,p.id as p_id,`p`.`value`,`p`.`is_where`,`p`.`sku_unit`,`p`.`repeat_type`,`p`.`goods_id` FROM `yzn_goods_property` `p` LEFT JOIN `yzn_goods` `g` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `p`.`recommend` = 1 GROUP BY `p`.`goods_id`,`p`.`repeat_type` ORDER BY `p`.`sort` DESC,`g`.`id` DESC LIMIT 0,15 [ RunTime:0.003019s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods_category` [ RunTime:0.001215s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `hot_search` = 1 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000666s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `is_show` > 0 AND `status` = 1 ORDER BY `listorder` ASC [ RunTime:0.000484s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `id` IN (1,2,7,16,17,34,92,95,38) [ RunTime:0.000476s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 1 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000363s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 2 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000344s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 7 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000357s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 16 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000347s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 17 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000347s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` IN (34,92) AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000354s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` IN (95,38) AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000363s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 1 AND `status` = 1 [ RunTime:0.000319s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 1 [ RunTime:0.000317s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,1,%' ) [ RunTime:0.000342s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods` [ RunTime:0.001302s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (25,26,29,30,48,1) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001818s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 2 AND `status` = 1 [ RunTime:0.000374s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 2 [ RunTime:0.000344s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,2,%' ) [ RunTime:0.000324s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (42,43,2) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.002069s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 7 AND `status` = 1 [ RunTime:0.000362s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 7 [ RunTime:0.000328s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,7,%' ) [ RunTime:0.000377s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (10,14,32,33,49,50,51,52,53,54,55,56,57,58,96,97,7) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001074s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 16 AND `status` = 1 [ RunTime:0.000346s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 16 [ RunTime:0.000350s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,16,%' ) [ RunTime:0.000330s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (59,60,61,62,63,16) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001514s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 17 AND `status` = 1 [ RunTime:0.000376s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 17 [ RunTime:0.000493s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,17,%' ) [ RunTime:0.000350s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (40,64,65,66,67,68,100,17) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001369s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 34 AND `status` = 1 [ RunTime:0.000330s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 92 AND `status` = 1 [ RunTime:0.000324s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` IN (34,92) [ RunTime:0.000368s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,34,%' or arrparentid like '0,92,%' ) [ RunTime:0.000343s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (35,70,71,93,94,34,92) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001534s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 95 AND `status` = 1 [ RunTime:0.000346s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 38 AND `status` = 1 [ RunTime:0.000351s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` IN (95,38) [ RunTime:0.000362s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,38,%' or arrparentid like '0,95,%' ) [ RunTime:0.000327s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (47,74,75,76,77,78,79,80,81,82,83,99,95,38) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001681s ]
[ SQL ] SHOW COLUMNS FROM `yzn_article` [ RunTime:0.001515s ]
[ SQL ] SELECT `A`.*,`AD`.*,`C`.`catname` FROM `yzn_article` `A` INNER JOIN `yzn_article_data` `AD` ON `A`.`id`=`AD`.`did` INNER JOIN `yzn_category` `C` ON `C`.`id`=`A`.`catid` WHERE `A`.`id` = 6385 AND `A`.`status` = 1 LIMIT 1 [ RunTime:0.002095s ]
[ SQL ] SHOW COLUMNS FROM `yzn_category` [ RunTime:0.001027s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 35 AND `status` = 1 [ RunTime:0.000319s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` IN (36,37,38,39,40,41,42) AND `status` = 1 [ RunTime:0.000333s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 43 AND `status` = 1 [ RunTime:0.000301s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` IN (44,45,46,47,48,49,50) AND `status` = 1 [ RunTime:0.000357s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 6 AND `status` = 1 [ RunTime:0.000297s ]
[ DB ] CONNECT:[ UseTime:0.000479s ] mysql:host=127.0.0.1;port=3306;dbname=yzncms;charset=utf8
[ SQL ] SELECT `A`.`id`,`A`.`title` FROM `yzn_article` `A` INNER JOIN `yzn_category` `C` ON `C`.`id`=`A`.`catid` WHERE `A`.`status` = 1 AND `A`.`catid` IN (35,36,37,38,39,40,41,42) ORDER BY `A`.`flag` DESC,`A`.`listorder` ASC,`A`.`updatetime` DESC [ RunTime:0.029275s ]
[ SQL ] UPDATE `yzn_article` SET `hits` = `hits` + 1 WHERE `id` = 6385 [ RunTime:0.000537s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 10 AND `p_c_id` = 41 ORDER BY `listorder` ASC LIMIT 4 [ RunTime:0.000654s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 38 AND `a`.`status` = 1 [ RunTime:0.003313s ]
[ SQL ] SELECT `a`.*,`c`.`catname` FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 38 AND `a`.`status` = 1 ORDER BY `flag` DESC,`listorder` ASC,`updatetime` DESC LIMIT 0,6 [ RunTime:0.006820s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 37 AND `a`.`status` = 1 [ RunTime:0.003497s ]
[ SQL ] SELECT `a`.*,`c`.`catname` FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 37 AND `a`.`status` = 1 ORDER BY `flag` DESC,`listorder` ASC,`updatetime` DESC LIMIT 0,6 [ RunTime:0.007291s ]