酯香香

大连化物所研发出甲醇生物转化"细胞工厂"

阅读量：4

发布时间：2021-08-19

来源：酯香香化学采购网

　　跟人类一样，真菌也有口味之别。汉逊酵母喜欢“吃”甲醇，但它却是个只吃饭不干活“懒汉”。中科院大连化物所研究员周雍进对这个“懒汉”情有独钟，他认为只要稍加引导，“懒汉”也能变“巧妇”，代谢产生人类需要的化学物质。

　　近日，周雍进团队实现了汉逊酵母的高效精确基因编辑，建立了高同源重组效率的精准基因组编辑工具，初步揭示了同源重组和非同源末端连接修复方式的竞争机制，构建了汉逊酵母“细胞工厂”，实现以甲醇和葡萄糖为原料合成脂肪醇的过程。相关研究内容发表在《交叉科学》上。

　　“缺乏遗传操作工具、同源重组效率低是难以改造汉逊酵母‘懒惰’特性的关键。”周雍进表示，研究团队希望用基因编辑手段剔除掉汉逊酵母基因中阻碍代谢产物合成的部分，增加有助产物合成的基因，让其高产起来。

　　基于CRISPR/Cas9的基因编辑技术，研究团队通过优化Cas9蛋白和sgRNA表达，成功将基因编辑效率提高至90%以上。在此基础上，揭示了同源重组和非同源末端连接的竞争关系，为此抑制了同源末端连接关键基因KU80的表达，增强了同源重组修复蛋白Rad51、Rad52、Sae2表达，成功在汉逊酵母中实现基因无缝敲除、大片段整合以及多片段重组，最终将同源重组效率提高到60%～70%。“改头换面”的汉逊酵母表现优异，以甲醇和葡萄糖为底物发酵生产出脂肪醇。

　　据了解，脂肪醇是一种重要的化学品，洗涤剂、化妆品、医药、润滑油等的制造都离不开它。可以说，汉逊酵母就像一座以甲醇和葡萄糖为底物生产脂肪醇的“细胞工厂”。与工业上使用羰基合成或油脂氢解法制脂肪醇的方法相比，汉逊酵母所需的底物甲醇和葡萄糖的来源更广泛，同时整个生产过程更环保。

　　周雍进表示，高同源重组效率的精准基因组编辑工具具有广谱性，有望应用于多种真菌和化学品的生产中。接下来，团队将进一步研究提高脂肪醇产率的方法，以及该工具在更多复杂化学品生产中的作用。

内容来源于酯香香化学采购网

下一篇：基本面不断改善甲醇价格有望维持强势

上一篇：再签4万吨！双良节能&通威再度合作

版权申明：

1.凡本网注明“来源：酯香香化学采购网”的所有文字、图片、音频和视频文件，版权均为有料网所有。如需转载请与客服电话：4008-938-935联系。任何媒体、网站或个人转载使用时须注明来源“有料网”，违反者本网将追究法律责任。
2.本网转载并注明其他来源的稿件，均来自互联网或业内投稿人士，版权属于原版权人。转载请保留稿件来源及作者，禁止擅自篡改违者自负版权法律责任。
3.本网评论版权中各网友的评论只代表网友个人观点，不代表本站的观点或立场。

产品品鉴

生物医用酯

酯类增塑剂

基础油脂

化工资讯

我国天然气产量连续3年增产超100亿立方米 2020中国（河北）国际橡胶塑料产业博览会火热启动 2020上海国际金属加工液、润滑油展览会火热启动 OPEC迎十年最大减产明年油价或继续承压 “智能+绿色”成化工教育重要方向污水处理迎来快速增长期化工人才需求上涨11.5%

行业新闻

加拿大油菜籽压榨步伐强劲同比增长近5个百分点 Nornickel将污染严重的铜冶炼厂关闭以推动环保财务省：日本11月精炼铜量同比增长37.4 中国主要铜冶炼厂下调2021年一季度的TC/RC 托管太钢集团，宝武系A股方阵扩容至10家中国进口澳大利亚铜精矿数量降至2016年以来最低水平

基本文件流程错误 SQL 调试

/www/dev/zhihuagong/public/index.php ( 1.51 KB )
/www/dev/zhihuagong/thinkphp/base.php ( 1.71 KB )
/www/dev/zhihuagong/thinkphp/library/think/Loader.php ( 12.70 KB )
/www/dev/zhihuagong/vendor/composer/autoload_static.php ( 21.91 KB )
/www/dev/zhihuagong/thinkphp/library/think/Error.php ( 4.00 KB )
/www/dev/zhihuagong/vendor/psr/log/Psr/Log/LoggerInterface.php ( 3.04 KB )
/www/dev/zhihuagong/thinkphp/library/think/Container.php ( 17.20 KB )
/www/dev/zhihuagong/thinkphp/library/think/App.php ( 27.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/Env.php ( 2.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/Config.php ( 9.71 KB )
/www/dev/zhihuagong/thinkphp/convention.php ( 11.74 KB )
/www/dev/zhihuagong/application/tags.php ( 1.08 KB )
/www/dev/zhihuagong/thinkphp/library/think/Hook.php ( 5.58 KB )
/www/dev/zhihuagong/application/common/behavior/Config.php ( 1.68 KB )
/www/dev/zhihuagong/application/common.php ( 32.83 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Request.php ( 6.32 KB )
/www/dev/zhihuagong/thinkphp/library/think/Facade.php ( 3.44 KB )
/www/dev/zhihuagong/thinkphp/library/think/Request.php ( 57.54 KB )
/www/dev/zhihuagong/application/function.php ( 0.68 KB )
/www/dev/zhihuagong/thinkphp/helper.php ( 20.40 KB )
/www/dev/zhihuagong/config/addons.php ( 1.33 KB )
/www/dev/zhihuagong/config/app.php ( 6.17 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Env.php ( 1.17 KB )
/www/dev/zhihuagong/config/cache.php ( 1.59 KB )
/www/dev/zhihuagong/config/captcha.php ( 0.96 KB )
/www/dev/zhihuagong/config/constant.php ( 1.33 KB )
/www/dev/zhihuagong/config/cookie.php ( 1.12 KB )
/www/dev/zhihuagong/config/database.php ( 2.36 KB )
/www/dev/zhihuagong/config/info.php ( 0.82 KB )
/www/dev/zhihuagong/config/log.php ( 1.01 KB )
/www/dev/zhihuagong/config/session.php ( 1.06 KB )
/www/dev/zhihuagong/config/template.php ( 2.24 KB )
/www/dev/zhihuagong/config/token.php ( 1.43 KB )
/www/dev/zhihuagong/config/trace.php ( 0.84 KB )
/www/dev/zhihuagong/config/version.php ( 0.92 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-php80/bootstrap.php ( 1.46 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-mbstring/bootstrap.php ( 7.07 KB )
/www/dev/zhihuagong/vendor/symfony/var-dumper/Resources/functions/dump.php ( 0.79 KB )
/www/dev/zhihuagong/vendor/symfony/deprecation-contracts/function.php ( 0.98 KB )
/www/dev/zhihuagong/vendor/ralouphie/getallheaders/src/getallheaders.php ( 1.60 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/psr7/src/functions_include.php ( 0.15 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/psr7/src/functions.php ( 13.09 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-intl-normalizer/bootstrap.php ( 0.71 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-php72/bootstrap.php ( 1.89 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/promises/src/functions_include.php ( 0.16 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/promises/src/functions.php ( 9.89 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-intl-idn/bootstrap.php ( 4.52 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/guzzle/src/functions_include.php ( 0.16 KB )
/www/dev/zhihuagong/vendor/guzzlehttp/guzzle/src/functions.php ( 9.70 KB )
/www/dev/zhihuagong/vendor/symfony/polyfill-ctype/bootstrap.php ( 1.56 KB )
/www/dev/zhihuagong/vendor/khanamiryan/qrcode-detector-decoder/lib/common/customFunctions.php ( 1.75 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/helpers.php ( 3.61 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/alias.php ( 1.21 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Arrayable.php ( 0.19 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Jsonable.php ( 0.24 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Contracts/Support/Htmlable.php ( 0.19 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Arr.php ( 16.36 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Traits/Macroable.php ( 2.63 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Collection.php ( 33.51 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Traits/EnumeratesValues.php ( 26.07 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/Enumerable.php ( 23.81 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/HigherOrderCollectionProxy.php ( 1.40 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/HigherOrderWhenProxy.php ( 1.30 KB )
/www/dev/zhihuagong/vendor/tightenco/collect/src/Collect/Support/LazyCollection.php ( 33.64 KB )
/www/dev/zhihuagong/vendor/overtrue/pinyin/src/const.php ( 0.49 KB )
/www/dev/zhihuagong/vendor/topthink/think-captcha/src/helper.php ( 1.54 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Route.php ( 3.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/Route.php ( 25.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Domain.php ( 7.08 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleGroup.php ( 16.45 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Rule.php ( 28.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleItem.php ( 8.84 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/RuleName.php ( 3.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Validate.php ( 4.83 KB )
/www/dev/zhihuagong/thinkphp/library/think/Validate.php ( 43.93 KB )
/www/dev/zhihuagong/vendor/topthink/think-helper/src/helper.php ( 7.35 KB )
/www/dev/zhihuagong/vendor/overtrue/wechat/src/Kernel/Support/Helpers.php ( 2.54 KB )
/www/dev/zhihuagong/vendor/overtrue/wechat/src/Kernel/Helpers.php ( 1.89 KB )
/www/dev/zhihuagong/thinkphp/library/think/Db.php ( 7.67 KB )
/www/dev/zhihuagong/thinkphp/library/think/Lang.php ( 7.38 KB )
/www/dev/zhihuagong/thinkphp/library/think/Log.php ( 8.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/log/driver/File.php ( 8.97 KB )
/www/dev/zhihuagong/thinkphp/lang/zh-cn.php ( 12.58 KB )
/www/dev/zhihuagong/route/route.php ( 0.67 KB )
/www/dev/zhihuagong/route/route_cms.php ( 2.89 KB )
/www/dev/zhihuagong/route/router_api.php ( 0.84 KB )
/www/dev/zhihuagong/route/router_h5.php ( 0.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Config.php ( 1.52 KB )
/www/dev/zhihuagong/extend/libs/Cache_factory.php ( 2.92 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Cache.php ( 2.06 KB )
/www/dev/zhihuagong/thinkphp/library/think/Cache.php ( 3.27 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/driver/File.php ( 7.99 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/Driver.php ( 8.64 KB )
/www/dev/zhihuagong/application/common/behavior/InitHook.php ( 1.47 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Hook.php ( 1.46 KB )
/www/dev/zhihuagong/addons/phpmailer/Phpmailer.php ( 2.00 KB )
/www/dev/zhihuagong/extend/sys/Addons.php ( 6.49 KB )
/www/dev/zhihuagong/thinkphp/library/think/View.php ( 5.74 KB )
/www/dev/zhihuagong/thinkphp/library/think/view/driver/Think.php ( 6.12 KB )
/www/dev/zhihuagong/thinkphp/library/think/Template.php ( 47.31 KB )
/www/dev/zhihuagong/thinkphp/library/think/template/driver/File.php ( 2.29 KB )
/www/dev/zhihuagong/application/cms/behavior/Hooks.php ( 1.10 KB )
/www/dev/zhihuagong/extend/sys/Hooks.php ( 2.48 KB )
/www/dev/zhihuagong/application/cms/function.php ( 10.81 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/dispatch/Module.php ( 5.19 KB )
/www/dev/zhihuagong/thinkphp/library/think/route/Dispatch.php ( 9.26 KB )
/www/dev/zhihuagong/application/cms/config/paginate.php ( 0.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/Middleware.php ( 5.34 KB )
/www/dev/zhihuagong/thinkphp/library/think/Cookie.php ( 7.35 KB )
/www/dev/zhihuagong/thinkphp/library/think/Session.php ( 14.13 KB )
/www/dev/zhihuagong/thinkphp/library/think/Debug.php ( 7.57 KB )
/www/dev/zhihuagong/application/cms/controller/Article.php ( 3.50 KB )
/www/dev/zhihuagong/application/cms/controller/Cmsbase.php ( 11.29 KB )
/www/dev/zhihuagong/application/member/controller/MemberBase.php ( 2.70 KB )
/www/dev/zhihuagong/application/common/controller/Homebase.php ( 2.38 KB )
/www/dev/zhihuagong/application/common/controller/Base.php ( 0.87 KB )
/www/dev/zhihuagong/thinkphp/library/think/Controller.php ( 7.53 KB )
/www/dev/zhihuagong/thinkphp/library/traits/controller/Jump.php ( 4.76 KB )
/www/dev/zhihuagong/application/member/service/User.php ( 13.62 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Cookie.php ( 1.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Url.php ( 1.13 KB )
/www/dev/zhihuagong/thinkphp/library/think/Url.php ( 13.22 KB )
/www/dev/zhihuagong/thinkphp/library/think/cache/driver/Redis.php ( 7.24 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Connection.php ( 58.93 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/connector/Mysql.php ( 6.22 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/builder/Mysql.php ( 5.85 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Builder.php ( 36.76 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Query.php ( 105.00 KB )
/www/dev/zhihuagong/application/cms/service/GoodsService.php ( 14.62 KB )
/www/dev/zhihuagong/application/cms/service/Base.php ( 0.62 KB )
/www/dev/zhihuagong/application/common/model/Goods.php ( 0.08 KB )
/www/dev/zhihuagong/thinkphp/library/think/Model.php ( 28.52 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/Attribute.php ( 17.64 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/RelationShip.php ( 20.60 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/ModelEvent.php ( 6.04 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/TimeStamp.php ( 2.58 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/concern/Conversion.php ( 7.84 KB )
/www/dev/zhihuagong/application/cms/service/ComponentsService.php ( 12.91 KB )
/www/dev/zhihuagong/application/admin/model/Config.php ( 3.34 KB )
/www/dev/zhihuagong/application/links/model/Links.php ( 0.84 KB )
/www/dev/zhihuagong/application/admin/model/Poster.php ( 0.07 KB )
/www/dev/zhihuagong/application/admin/model/Base.php ( 4.15 KB )
/www/dev/zhihuagong/thinkphp/library/think/facade/Session.php ( 2.05 KB )
/www/dev/zhihuagong/application/admin/model/GoodsCategory.php ( 0.09 KB )
/www/dev/zhihuagong/thinkphp/library/think/exception/ErrorException.php ( 1.68 KB )
/www/dev/zhihuagong/thinkphp/library/think/Exception.php ( 1.61 KB )
/www/dev/zhihuagong/thinkphp/library/think/exception/Handle.php ( 8.96 KB )
/www/dev/zhihuagong/thinkphp/library/think/db/Expression.php ( 1.11 KB )
/www/dev/zhihuagong/thinkphp/library/think/model/Collection.php ( 3.23 KB )
/www/dev/zhihuagong/thinkphp/library/think/Collection.php ( 13.60 KB )
/www/dev/zhihuagong/runtime/temp/b275df7f7078649353258fc8c4a5003e.php ( 37.86 KB )
/www/dev/zhihuagong/thinkphp/library/think/Response.php ( 9.68 KB )
/www/dev/zhihuagong/thinkphp/library/think/debug/Html.php ( 4.02 KB )

[ DB ] CONNECT:[ UseTime:0.000258s ] mysql:host=127.0.0.1;port=3306;dbname=yzncms;charset=utf8
[ SQL ] SHOW COLUMNS FROM `yzn_poster` [ RunTime:0.001371s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 99 AND `p_c_id` = 52 AND `status` = 1 LIMIT 1 [ RunTime:0.000350s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 99 AND `p_c_id` = 55 AND `status` = 1 LIMIT 1 [ RunTime:0.000260s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods_property` [ RunTime:0.001338s ]
[ SQL ] SELECT `g`.`name`,`p`.`price`,`p`.`unit`,`p`.`type`,`p`.`thumb`,p.id as p_id,`p`.`value`,`p`.`is_where`,`p`.`sku_unit`,`p`.`repeat_type`,`p`.`goods_id` FROM `yzn_goods_property` `p` LEFT JOIN `yzn_goods` `g` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `p`.`recommend` = 1 GROUP BY `p`.`goods_id`,`p`.`repeat_type` ORDER BY `p`.`sort` DESC,`g`.`id` DESC LIMIT 0,15 [ RunTime:0.002412s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods_category` [ RunTime:0.000984s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `hot_search` = 1 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000352s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `is_show` > 0 AND `status` = 1 ORDER BY `listorder` ASC [ RunTime:0.000352s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `id` IN (1,2,7,16,17,34,92,95,38) [ RunTime:0.000249s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 1 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000185s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 2 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000162s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 7 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000161s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 16 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000166s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 17 AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000173s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` IN (34,92) AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000168s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` IN (95,38) AND `status` = 1 ORDER BY `listorder` ASC,`id` DESC [ RunTime:0.000160s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 1 AND `status` = 1 [ RunTime:0.000152s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 1 [ RunTime:0.000162s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,1,%' ) [ RunTime:0.000164s ]
[ SQL ] SHOW COLUMNS FROM `yzn_goods` [ RunTime:0.000639s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (25,26,29,30,48,1) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001062s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 2 AND `status` = 1 [ RunTime:0.000225s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 2 [ RunTime:0.000201s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,2,%' ) [ RunTime:0.000210s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (42,43,2) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.001007s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 7 AND `status` = 1 [ RunTime:0.000209s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 7 [ RunTime:0.000215s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,7,%' ) [ RunTime:0.000197s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (10,14,32,33,49,50,51,52,53,54,55,56,57,58,96,97,7) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.000997s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 16 AND `status` = 1 [ RunTime:0.000211s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 16 [ RunTime:0.000205s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,16,%' ) [ RunTime:0.000194s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (59,60,61,62,63,16) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.000923s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 17 AND `status` = 1 [ RunTime:0.000225s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` = 17 [ RunTime:0.000229s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,17,%' ) [ RunTime:0.000200s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (40,64,65,66,67,68,100,17) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.000936s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 34 AND `status` = 1 [ RunTime:0.000238s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 92 AND `status` = 1 [ RunTime:0.000189s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` IN (34,92) [ RunTime:0.000227s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,34,%' or arrparentid like '0,92,%' ) [ RunTime:0.000247s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (35,70,71,93,94,34,92) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.000978s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 95 AND `status` = 1 [ RunTime:0.000214s ]
[ SQL ] SELECT `id`,`catname` FROM `yzn_goods_category` WHERE `pid` = 38 AND `status` = 1 [ RunTime:0.000192s ]
[ SQL ] SELECT * FROM `yzn_goods_category` WHERE `status` = 1 AND `id` IN (95,38) [ RunTime:0.000256s ]
[ SQL ] SELECT `id` FROM `yzn_goods_category` WHERE `status` = 1 AND ( arrparentid like '0,38,%' or arrparentid like '0,95,%' ) [ RunTime:0.000209s ]
[ SQL ] SELECT `g`.`name`,p.id as p_id,`p`.`price`,`p`.`type`,`p`.`thumb`,`p`.`unit`,`p`.`is_where`,`p`.`goods_id`,`p`.`repeat_type` FROM `yzn_goods` `g` LEFT JOIN `yzn_goods_property` `p` ON `p`.`goods_id`=`g`.`id` WHERE `p`.`status` = 1 AND `p`.`is_del` = 0 AND `g`.`is_del` = 0 AND `p`.`is_where` LIKE '%1%' AND `g`.`cate_id` IN (47,74,75,76,77,78,79,80,81,82,83,99,95,38) AND `p`.`hot_sale` = '1' GROUP BY `p`.`repeat_type`,`p`.`goods_id` ORDER BY `p`.`sort` DESC,`p`.`id` DESC LIMIT 0,10 [ RunTime:0.000949s ]
[ SQL ] SHOW COLUMNS FROM `yzn_article` [ RunTime:0.000858s ]
[ SQL ] SELECT `A`.*,`AD`.*,`C`.`catname` FROM `yzn_article` `A` INNER JOIN `yzn_article_data` `AD` ON `A`.`id`=`AD`.`did` INNER JOIN `yzn_category` `C` ON `C`.`id`=`A`.`catid` WHERE `A`.`id` = 2324 AND `A`.`status` = 1 LIMIT 1 [ RunTime:0.000583s ]
[ SQL ] SHOW COLUMNS FROM `yzn_category` [ RunTime:0.000598s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 35 AND `status` = 1 [ RunTime:0.000197s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` IN (36,37,38,39,40,41,42) AND `status` = 1 [ RunTime:0.000187s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 43 AND `status` = 1 [ RunTime:0.000189s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` IN (44,45,46,47,48,49,50) AND `status` = 1 [ RunTime:0.000184s ]
[ SQL ] SELECT `id` FROM `yzn_category` WHERE `parentid` = 6 AND `status` = 1 [ RunTime:0.000167s ]
[ DB ] CONNECT:[ UseTime:0.000259s ] mysql:host=127.0.0.1;port=3306;dbname=yzncms;charset=utf8
[ SQL ] SELECT `A`.`id`,`A`.`title` FROM `yzn_article` `A` INNER JOIN `yzn_category` `C` ON `C`.`id`=`A`.`catid` WHERE `A`.`status` = 1 AND `A`.`catid` IN (35,36,37,38,39,40,41,42) ORDER BY `A`.`flag` DESC,`A`.`listorder` ASC,`A`.`updatetime` DESC [ RunTime:0.021022s ]
[ SQL ] UPDATE `yzn_article` SET `hits` = `hits` + 1 WHERE `id` = 2324 [ RunTime:0.000271s ]
[ SQL ] SELECT * FROM `yzn_poster` WHERE `cate_id` = 10 AND `p_c_id` = 41 ORDER BY `listorder` ASC LIMIT 4 [ RunTime:0.000341s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 38 AND `a`.`status` = 1 [ RunTime:0.002697s ]
[ SQL ] SELECT `a`.*,`c`.`catname` FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 38 AND `a`.`status` = 1 ORDER BY `flag` DESC,`listorder` ASC,`updatetime` DESC LIMIT 0,6 [ RunTime:0.004665s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 37 AND `a`.`status` = 1 [ RunTime:0.002567s ]
[ SQL ] SELECT `a`.*,`c`.`catname` FROM `yzn_article` `a` INNER JOIN `yzn_category` `c` ON `a`.`catid`=`c`.`id` WHERE `catid` = 37 AND `a`.`status` = 1 ORDER BY `flag` DESC,`listorder` ASC,`updatetime` DESC LIMIT 0,6 [ RunTime:0.004914s ]